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Table 1 Estimated storage time in days during desiccation on two matrices to achieve target CFU of 1000 or 1

From: Desiccation induces viable but Non-Culturable cells in Sinorhizobium meliloti 1021

 

Matrix*

 

Nitrocellulose

Ottawa Sand

Alfalfa seed

Initial reduction (IR†)

41.6 ± 7.5

33.0 ± 12.8

(6.2 ± 2.2) × 103

Target CFU (Nf‡)

1

1000

1

1000

1

1000

Initial CFU (N0§)

   1 × 107

128 ± 2

60 ± 2

134 ± 5

66 ± 5

78 ± 1

10 ± 1

   2 × 109

181 ± 2

113 ± 2

187 ± 5

118 ± 5

131 ± 1

62 ± 1

  1. (*) Error represent the Standard Error of the Mean with n = 6
  2. (†) IR = Initial Reduction (Nt0-5 = N0/Nt = 5)
  3. (‡) Nf = Target CFU. Complete loss (< 1 CFU), French Canadian Standard = 1000 CFU (Smith 1992)
  4. (§) N0 = Initial CFU