Table 2 Primers used in this study
From: Contributions of mutations in acrR and marR genes to organic solvent tolerance in Escherichia coli
Primer | Sequence (5′ to 3′) | Positionsa |
|---|---|---|
acrR-F | AAACCCATTGCTGCGTTTAT | − 90 to − 71 bp of acrR |
acrR-R | AAACCGCAAGAATATCACGA | + 711 to + 730 bp of acrR |
marR-F | CTGTTCATGTTGCCTGCCAG | − 321 to − 302 bp of marR |
marR-R | CAGTCCAAAATGCTATGAATGG | + 482 to + 503 bp of marR |
soxR-F | TTTCTGATGGGACATAAATCTGCC | − 100 to − 77 bp of soxR |
soxR-R | TGTGTTGACGTCGGGGGAAA | + 537 to + 556 bp of soxR |
acrA-F | CCAATTTGAAATCGGACACTCG | − 32 to − 11 bp of acrA |
acrA-R | GCATGTCTTAACGGCTCCTG | + 1200 to + 1219 bp of acrA |
acrR-rpsL-neo-F | GCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAG GGCCTGGTGATGATGGCGGGATCG | − 376 to − 327 bp of acrR (indicated by italics),and − 138 to − 115 bp of rpsL in the rpsL-neocassette (underlined) |
acrR-rpsL-neo-R | GCGATCGATTTTATCGAGGGTGGCTAATGTATCTGTCAGATCCTGCTGCA TCAGAAGAACTCGTCAAGAAGGCG | + 974 to + 1023 bp of acrR (indicated by italics),and + 750 to + 773 bp of neo in the rpsL-neocassette (underlined) |
marR-rpsL-neo-F | AAACCGATAAACGCGACGATTAAGCCGCCTGCAATTCGCAGACCGGGAAT GGCCTGGTGATGATGGCGGGATCG | − 477 to − 428 bp of marR (indicated by italics),and − 138 to − 115 bp of rpsL in the rpsL-neocassette (underlined) |
marR-rpsL-neo-R | AAGAGAATAAGCGCAGCTGCTATTGCGGATGAAAGTGGTTTCATGATTGC TCAGAAGAACTCGTCAAGAAGGCG | + 863 to + 912 bp of marR (indicated by italics),and + 750 to + 773 bp of neo in the rpsL-neocassette (underlined) |
Repair-acrRIS-F | TGATCGTACTCTTGCTTACTGAT | − 575 to − 553 bp of acrR |
Repair-acrRIS-R | ATCGTTTTGTGCGTTTTGCAAAT | + 1202 to + 1224 bp of acrR |
Repair-marR-F | TTTTCGCCTCCGGTGAATCA | − 527 to − 508 bp of marR |
Repair-marR-R | AACTGGCTGCGTGGTTTGTT | + 933 to + 952 bp of marR |