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Table 3 Confirmation of appearance of amino acids in comprehensively mutated CBM in 88 samples

From: Construction of a novel selection system for endoglucanases exhibiting carbohydrate-binding modules optimized for biomass using yeast cell-surface engineering

A.A. number; 18 A.A. number; 23 A.A. number; 26 A.A. number; 27
A.A. Rate A.A. Rate A.A. Rate A.A. Rate
T 10 (11.4%) S 12 (13.6%) L 10 (11.4%) L 11 (12.5%)
S 9 (10.2%) G 9 (10.2%) V 8 (9.1%) R 9 (10.2%)
A 9 L 7 (8.0%) S 8 S 8 (9.1%)
L 7 (8.0%) T 7 R 7 (8.0%) T 7 (8.0%)
P 7 R 7 W 6 (6.8%) G 6 (6.8%)
F 6 (6.8%) A 6 (6.8%) I 5 (5.7%) C 5 (5.7%)
R 6 M 6 Stop 5 P 5
E 5 (5.7%) W 5 (5.75) G 5 W 4 (4.5%)
Y 4 (4.55) H 4 (4.5%) T 4 (4.5%) V 4
V 3 (3.4) Q 4 P 4 A 3 (3.4%)
Stop 3 N 3 (3.4%) Y 4 F 3
N 3 D 3 A 3 (3.4%) D 3
K 3 I 3 M 3 M 3
C 2 (2.3%) F 2 (2.3%) F 3 H 3
W 2 K 2 C 3 K 3
G 2 V 2 N 2 (23%) Stop 3
Q 2 P 2 E 2 I 2 (2.3%)
I 2 Y 2 Q 2 Q 2
D 2 E 1 (1.1%) K 1 (1.1%) Y 2
M 1 (1.1%) Stop 1 H 1 N 1 (1.1%)
     F 1 E 1
     D 1