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Table 3 Confirmation of appearance of amino acids in comprehensively mutated CBM in 88 samples

From: Construction of a novel selection system for endoglucanases exhibiting carbohydrate-binding modules optimized for biomass using yeast cell-surface engineering

A.A. number; 18

A.A. number; 23

A.A. number; 26

A.A. number; 27

A.A.

Rate

A.A.

Rate

A.A.

Rate

A.A.

Rate

T

10 (11.4%)

S

12 (13.6%)

L

10 (11.4%)

L

11 (12.5%)

S

9 (10.2%)

G

9 (10.2%)

V

8 (9.1%)

R

9 (10.2%)

A

9

L

7 (8.0%)

S

8

S

8 (9.1%)

L

7 (8.0%)

T

7

R

7 (8.0%)

T

7 (8.0%)

P

7

R

7

W

6 (6.8%)

G

6 (6.8%)

F

6 (6.8%)

A

6 (6.8%)

I

5 (5.7%)

C

5 (5.7%)

R

6

M

6

Stop

5

P

5

E

5 (5.7%)

W

5 (5.75)

G

5

W

4 (4.5%)

Y

4 (4.55)

H

4 (4.5%)

T

4 (4.5%)

V

4

V

3 (3.4)

Q

4

P

4

A

3 (3.4%)

Stop

3

N

3 (3.4%)

Y

4

F

3

N

3

D

3

A

3 (3.4%)

D

3

K

3

I

3

M

3

M

3

C

2 (2.3%)

F

2 (2.3%)

F

3

H

3

W

2

K

2

C

3

K

3

G

2

V

2

N

2 (23%)

Stop

3

Q

2

P

2

E

2

I

2 (2.3%)

I

2

Y

2

Q

2

Q

2

D

2

E

1 (1.1%)

K

1 (1.1%)

Y

2

M

1 (1.1%)

Stop

1

H

1

N

1 (1.1%)

    

F

1

E

1

    

D

1