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Table 2 Primers to construct the comprehensively mutated CBM and to confirm the mutated CBM-sequences

From: Construction of a novel selection system for endoglucanases exhibiting carbohydrate-binding modules optimized for biomass using yeast cell-surface engineering

Primer Sequence
pEGout F 5’-CGTAGGTCCGCTCCAACCAATACC-3’
pEGout R 5’-CTCAATCCTTATTATGCGCAATGTATTCCG-3’
NNK primer 5’-TTGGAGCGGACCTACGNNKTGTGCTCCTGGCNNKGCTTCTNNKNNKCTCAATCCTTATTATGCGCAATGT-3’
NNK rev 5’-ACATTGCGCATAATAAGGATTGAG-3’
EGct F1 5’-CCAGTGTGGAGGTATTGGTTGGAGCGGACCTACG-3’
EGct R1 5’-GTCGAAGTGGTGATAGTAGTGGCTCCCGGAATACATTGCGCATAATAAGGATTGAG-3’
EGct F2 5’-ATAGATCTCAGCAGACTGTCTGGGGCCAGTGTGGAGGTATTGG-3’
EGct R2 5’-GCACGCGTGGTGGTGGTTGGACCGGATGGTGGCCGGGTCGAAGTGGTGATAGTAGTGGC-3’
CHK 5’-CATGCAACTGTTCAATTTGCCATTG-3’
EGII360bp rev 5’-AGGATAAACCTTCGAGGTAACGCAAGTGCC-3’