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Table 2 Primers to construct the comprehensively mutated CBM and to confirm the mutated CBM-sequences

From: Construction of a novel selection system for endoglucanases exhibiting carbohydrate-binding modules optimized for biomass using yeast cell-surface engineering

Primer

Sequence

pEGout F

5’-CGTAGGTCCGCTCCAACCAATACC-3’

pEGout R

5’-CTCAATCCTTATTATGCGCAATGTATTCCG-3’

NNK primer

5’-TTGGAGCGGACCTACGNNKTGTGCTCCTGGCNNKGCTTCTNNKNNKCTCAATCCTTATTATGCGCAATGT-3’

NNK rev

5’-ACATTGCGCATAATAAGGATTGAG-3’

EGct F1

5’-CCAGTGTGGAGGTATTGGTTGGAGCGGACCTACG-3’

EGct R1

5’-GTCGAAGTGGTGATAGTAGTGGCTCCCGGAATACATTGCGCATAATAAGGATTGAG-3’

EGct F2

5’-ATAGATCTCAGCAGACTGTCTGGGGCCAGTGTGGAGGTATTGG-3’

EGct R2

5’-GCACGCGTGGTGGTGGTTGGACCGGATGGTGGCCGGGTCGAAGTGGTGATAGTAGTGGC-3’

CHK

5’-CATGCAACTGTTCAATTTGCCATTG-3’

EGII360bp rev

5’-AGGATAAACCTTCGAGGTAACGCAAGTGCC-3’