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Table 1 Comparison of CBM amino-acid sequences of EG in family 1 CBMs (92 samples)

From: Construction of a novel selection system for endoglucanases exhibiting carbohydrate-binding modules optimized for biomass using yeast cell-surface engineering

No.

A.A.

remarks column

5

W

W(61%), Y(39%)

6

G

G(91%), QA(3%), Y(2%)

7

Q

Unchanging

8

C

Unchanging

9

G

Unchanging

10

G

Unchanging

11

I

I(52%), Q(20%), S(14%), N(7%), T(4%), ARL(1%)

12

G

G(92%), N(9%), S(1%)

13

W

W(74%), Y(23%), F(3%)

14

S

T(52%), S(42%), N(3%), IK(1%)

15

G

Unchanging

16

P

P(74%), A(17%), S(4%), L(3%), Q(2%)

17

T

Unchanging

18

N

T(28%), N(13%), S(12%), AV(11%), QA(5%), RECT(3%), DKI(1%)

19

C

Unchanging

20

A

A(49%), V(36%), Q(5%), ET(4%), D(2%)

21

P

S(66%), A(21%), P(8%), T(4%), G(1%)

22

G

G(86%), P(13%), S(1%)

23

S

S(35%), A(25%), T(17%), Y(12%), FNL(3%), WG(1%)

24

A

T(67%), A(14%), V(9%), C(5%), S(3%), K(2%)

25

C

Uchanging

26

S

T(27%), Q(24%), S(22%), V(10%), H(8%), K(4%), AE(2%), M(1%)

27

T

V(38%), T(20%), K(17%), S(8%), Y(5%), AE(4%), IRQH(1%)

28

L

L(53%), Q(13%), YV(8%), I(6%), S(5%), T(3%), GAWM(1%)

29

N

N(97%), S(2%), G(1%)

30

P

P(56%), D(25%), AS(5%), QE(4%)

31

Y

Y(75%), W(22%), F(2%), A(1%)

32

Y

Y(99%), H(1%)

33

A

S(71%), A(20%), Y(4%), H(3%), F(2%)

34

Q

Uchanging

35

C

Uchanging

  1. Amino-acid numbers (No.) and sequences (A.A.; 1-letter-notation) of the CBM of EG; remarks column, the results of amino-acid comparisons in family 1 CBM; underline: the flexible residue (the conservation < 40%).