Fluorescence observation of RTSH yeast, harboring EG with CBM having RTSH mutation, CBH, and BG, after immunofluorescence labeling. Yeast cells were labeled with the following antibodies: mouse monoclonal anti-FLAG M2 antibody for EG (upper column), mouse monoclonal StrepMAB-Classic antibody for CBH (middle column), mouse monoclonal anti-RGS-His antibody for BG (lower column), and Alexa Fluor 488 anti-mouse immunoglobulin. The left column represents RTSH yeast and the right column represents yeast harboring pRS423, pRS425, and pRS426 (control vectors). Phase-contrast micrographs are presented in the left column and fluorescence micrographs are presented in the right column. The scale bar is 5 μm.