Figure 1From: Immunological characterization of recombinant Salmonella enterica serovar Typhi FliC protein expressed in Escherichia coliSchematic presentation of the pET28c+ vector construct (6887 bp) containing the fli C gene responsible for the expression of rFliC designated as pGRP-1. Figure 1B: Expression of the purified rFliC by SDS-PAGE. Lanes-1, 2 &3: A single band of purified rFliC (different amount of protein) observed after Ni2+-NTA affinity chromatography of cell lysate of IPTG induced E. coli BL21 (DE3) cells; & 4: Standard Protein Molecular Weight Marker (broad range).Back to article page