Phylogenetic tree based on the 16S rRNA gene sequences of denitrifying bacteria obtained using the FSC and APD isolation methods. Scale bar indicates the substitutions per site. Bootstrap values at the nodes are the percentages of 500 bootstrap replicates. Values more than 70% are indicated at branches. Bold letters indicate the sequence obtained in this study. Accession numbers are shown in parentheses. UNPF: FSC isolates; UNPA: APD isolates.