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Table 2 Amino acid sequences of tryptic peptide fragments of protein extracted from B. juncea using thin stillage

From: Biorefinery process for protein extraction from oriental mustard (Brassica juncea (L.) Czern.) using ethanol stillage

Subunit mass (kDa) Fragment sequence Calculated mass (m/z) Actual mass (m/z) Sequence assignment Position
14 EFQQAQQHLR 1155.58 1156.68 Allergen B. juncea 1-E 12-20
  IYQTATHLPR 1198.64 1199.75 Allergen B. juncea 1-E 100-109
18-20 GLPLEVISNGYQISPQEAR 2070.07 2071.21 Cruciferin 338-386
20-22 GLPLEVISNGYQISLEEAR 2087.09 2088.19 Cruciferin 66-84
  GLPLEVISNGYQISPQEAR 2070.07 2071.19 Cruciferin 368-386
34 CSGFAFER 972.41 973.49 Cruciferin 62-69
  VQGQFGVIRPPLR 1465.85 1466.94 Cruciferin 251-263
  IEVWDHHAPQLR 1499.76 1500.83 Cruciferin 50-61
55 GPFQVVRPPLR 1264.74 1265.79 Cruciferin 288-298
  VQGQFGVIRPPLR 1465.85 1466.91 Cruciferin 251-263
  IEVWDHHAPQLR 1499.76 1500.81 Cruciferin 50-61
  GLPLEVISNGYQISPQEAR 2070.07 2071.13 Cruciferin 420-438