Table 2 Amino acid sequences of tryptic peptide fragments of protein extracted from B. juncea using thin stillage
Subunit mass (kDa) | Fragment sequence | Calculated mass (m/z) | Actual mass (m/z) | Sequence assignment | Position |
|---|---|---|---|---|---|
14 | EFQQAQQHLR | 1155.58 | 1156.68 | Allergen B. juncea 1-E | 12-20 |
IYQTATHLPR | 1198.64 | 1199.75 | Allergen B. juncea 1-E | 100-109 | |
18-20 | GLPLEVISNGYQISPQEAR | 2070.07 | 2071.21 | Cruciferin | 338-386 |
20-22 | GLPLEVISNGYQISLEEAR | 2087.09 | 2088.19 | Cruciferin | 66-84 |
GLPLEVISNGYQISPQEAR | 2070.07 | 2071.19 | Cruciferin | 368-386 | |
34 | CSGFAFER | 972.41 | 973.49 | Cruciferin | 62-69 |
VQGQFGVIRPPLR | 1465.85 | 1466.94 | Cruciferin | 251-263 | |
IEVWDHHAPQLR | 1499.76 | 1500.83 | Cruciferin | 50-61 | |
55 | GPFQVVRPPLR | 1264.74 | 1265.79 | Cruciferin | 288-298 |
VQGQFGVIRPPLR | 1465.85 | 1466.91 | Cruciferin | 251-263 | |
IEVWDHHAPQLR | 1499.76 | 1500.81 | Cruciferin | 50-61 | |
GLPLEVISNGYQISPQEAR | 2070.07 | 2071.13 | Cruciferin | 420-438 |