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Table 5 Performance of C. acetobutylicum ATCC 824 and its transformants in 45‐h cultures performed without pH regulation

From: Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains

Performances C. acetobutylicum ATCC 824
WT (pMTL500E) (pFC002) (pFC005) (pFC006) (pFC007)
Glucose consumed [g/L] 65.0 (1.0) 61.6 (4.2) 50.1 (5.6) 59.3 (2.5) 67.9 (7.0)) 70.0 (1.9)
Acetic acid * [g/L] −1.6 (0.1) −1.8 (0.0) −1.3 (0.1) −2.0 (0.1) −1.2 (0.2) −1.4 (0.8)
Butyric acid [g/L] 1.1 (0.1) 1.1 (0.1) 1.0 (0.0) 1.1 (0.1) 0.6 (0.3) 0.8 (0.3)
Acetoin [g/L] 1.1 (0.1) 0.8 (0.0) 0.0 (0.0) (0.0 (0.0) 0.0 (0.0) 0.1 (0.1)
2,3-Butanediol ( D or L ) [g/L] 0.0 (0.0) 0.0 (0.0) 0.6 (0.0) 0.7 (0.2) 0.8 (0.2) 1.2 (0.6)
Ethanol [g/L] 0.9 (0.0) 1.2 (0.4) 1.6 (0.2) 0.9 (0.1) 3.1 (1.7) 1.5 (0.6)
Acetone [g/L] 7.6 (0.1) 7.2 (0.0) 0.7 (0.1) 0.0 (0.0) 0.3 (0.1) 0.1 (0.1)
Isopropanol [g/L] 0.1 (0.0) 0.1 (0.0) 6.0 (0.5) 8.2 (0.1) 8.0 (0.4) 8.8 (0.7)
Butanol [g/L] 11.6 (0.2) 12.6 (1.3) 9.5 (0.3) 12.1 (0.2) 13.4 (1.5) 13.7 (1.6)
Final solvents [g/L] 20.2 (0.3) 21.1 (1.6) 16.2 (0.9) 21.3 (0.2) 24.0 (2.5) 24.4 (2.8)
Final solvent yield [g A/IBE / g glc. ] 0.31 (0.01) 0.34 (0.00) 0.33 (0.02) 0.36 (0.01) 0.35 (0.00) 0.35 (0.04)
Productivity after 30 h [g/L h] 0.38 (0.00) 0.42 (0.01) 0.28 (0.02) 0.30 (0.00) 0.72 (0.04) 0.80 (0.11)
Carbone recovery [%] 89% 94% 102% 100% 105% 101%
  1. Cultures were performed in 1 L-working volume of CM1 containing 3.0 g/L ammonium acetate and 90 g/L glucose.
  2. All data are given as the mean of two or three fermentations the standard error to the mean is indicated in brackets.
  3. * a negative value (−) indicates that initial acetic acid from culture medium was partially consumed.