Table 5 Performance of C. acetobutylicum ATCC 824 and its transformants in 45‐h cultures performed without pH regulation
Performances | C. acetobutylicum ATCC 824 | |||||
|---|---|---|---|---|---|---|
WT | (pMTL500E) | (pFC002) | (pFC005) | (pFC006) | (pFC007) | |
Glucose consumed [g/L] | 65.0 (1.0) | 61.6 (4.2) | 50.1 (5.6) | 59.3 (2.5) | 67.9 (7.0)) | 70.0 (1.9) |
Acetic acid * [g/L] | −1.6 (0.1) | −1.8 (0.0) | −1.3 (0.1) | −2.0 (0.1) | −1.2 (0.2) | −1.4 (0.8) |
Butyric acid [g/L] | 1.1 (0.1) | 1.1 (0.1) | 1.0 (0.0) | 1.1 (0.1) | 0.6 (0.3) | 0.8 (0.3) |
Acetoin [g/L] | 1.1 (0.1) | 0.8 (0.0) | 0.0 (0.0) | (0.0 (0.0) | 0.0 (0.0) | 0.1 (0.1) |
2,3-Butanediol ( D or L ) [g/L] | 0.0 (0.0) | 0.0 (0.0) | 0.6 (0.0) | 0.7 (0.2) | 0.8 (0.2) | 1.2 (0.6) |
Ethanol [g/L] | 0.9 (0.0) | 1.2 (0.4) | 1.6 (0.2) | 0.9 (0.1) | 3.1 (1.7) | 1.5 (0.6) |
Acetone [g/L] | 7.6 (0.1) | 7.2 (0.0) | 0.7 (0.1) | 0.0 (0.0) | 0.3 (0.1) | 0.1 (0.1) |
Isopropanol [g/L] | 0.1 (0.0) | 0.1 (0.0) | 6.0 (0.5) | 8.2 (0.1) | 8.0 (0.4) | 8.8 (0.7) |
Butanol [g/L] | 11.6 (0.2) | 12.6 (1.3) | 9.5 (0.3) | 12.1 (0.2) | 13.4 (1.5) | 13.7 (1.6) |
Final solvents [g/L] | 20.2 (0.3) | 21.1 (1.6) | 16.2 (0.9) | 21.3 (0.2) | 24.0 (2.5) | 24.4 (2.8) |
Final solvent yield [g A/IBE / g glc. ] | 0.31 (0.01) | 0.34 (0.00) | 0.33 (0.02) | 0.36 (0.01) | 0.35 (0.00) | 0.35 (0.04) |
Productivity after 30 h [g/L h] | 0.38 (0.00) | 0.42 (0.01) | 0.28 (0.02) | 0.30 (0.00) | 0.72 (0.04) | 0.80 (0.11) |
Carbone recovery [%] | 89% | 94% | 102% | 100% | 105% | 101% |