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Table 4 Reduction activities of acetone and acetoin measured in cell-free extracts of C. beijerinckii NRRL B593 and C. acetobutylicum ATCC 824 transformants harbouring pMTL500E, pFC002 and pFC007

From: Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains

Strain Specific activity[μmol/min mgprotein]
acetone acetoin Control*
C. beijerinckii NRRL B593 0.063 0.069 0.015
C. acetobutylicum ATCC 824(pMTL500E) 0.002 0.007 0.003
C. acetobutylicum ATCC 824(pFC002) 0.152 0.069 0.009
C. acetobutylicum ATCC 824(pFC007) 0.076 0.118 0.018
  1. Cell biomass used for CFE was taken from 20 h cultures.
  2. Data are given as the mean of two replicates.
  3. * In control assays, substrate was replaced by HEPES buffer.