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Table 4 Reduction activities of acetone and acetoin measured in cell-free extracts of C. beijerinckii NRRL B593 and C. acetobutylicum ATCC 824 transformants harbouring pMTL500E, pFC002 and pFC007

From: Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains

Strain

Specific activity[μmol/min mgprotein]

acetone

acetoin

Control*

C. beijerinckii

NRRL B593

0.063

0.069

0.015

C. acetobutylicum

ATCC 824(pMTL500E)

0.002

0.007

0.003

C. acetobutylicum

ATCC 824(pFC002)

0.152

0.069

0.009

C. acetobutylicum

ATCC 824(pFC007)

0.076

0.118

0.018

  1. Cell biomass used for CFE was taken from 20 h cultures.
  2. Data are given as the mean of two replicates.
  3. * In control assays, substrate was replaced by HEPES buffer.