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Table 3 Performance of C. acetobutylicum ATCC 824 and its transformants in 45‐h cultures performed with pH regulation at 5.0

From: Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains

Performances C. beijerinckii 2 NRRL B5932 C. acetobutylicum ATCC 824
WT (pMTL500E) (pFC002) (pFC005) (pFC006) (pFC007)
Glucose consumed [g/L] 36.8 (1.7) 62.3 (0.4) 62.5 (2.4) 53.0 (7.9) 56.3 (2.2) 68.0 (4.3) 68.1 (0.1)
Acetic acid 1 [g/L] −1.7 (0.0) 0.3 (0.4) 0.4 (1.8) 1.8 (1.2) 1.2 (0.2) 1.0 (1.0) 0.0 (0.1)
Butyric acid [g/L] 0.3 (0.1) 1.7 (0.4) 1.3 (0.4) 2.6 (0.8) 2.5 (0.5) 2.1(1.6) 1.4 (0.0)
Acetoin [g/L] 0.0 (0.0) 1.1 (0.1) 0.8 (0.1) 0.0 (0.0) 0.1 (0.0) 0.0 (0.0) 0.1 (0.0)
2,3-Butanediol ( D or L ) [g/L] 0.0 (0.0) 0.0 (0.0) 0.0 (0.0) 0.5 (0.3) 0.5 (0.1) 0.6 (0.3) 0.6 (0.1)
Ethanol [g/L] 0.1 (0.0) 1.1 (0.2) 0.8 (0.2) 1.1 (0.3) 0.7 (0.0) 1.0 (0.3) 0.8 (0.7)
Acetone [g/L] 0.2 (0.0) 5.7 (0.0) 5.4 (1.0) 0.1 (0.1) 0.1 (0.0) 0.3 (0.2) 0.9 (0.9)
Isopropanol [g/L] 4.5 (0.3) 0.1 (0.1) 0.1 (0.1) 4.8 (0.8) 6.1 (0.1) 7.2 (0.5) 7.3 (0.3)
Butanol [g/L] 8.4 (1.9) 10.0 (1.5) 10.1 (1.5) 9.1 (1.6) 9.7 (0.3) 11.3 (0.8) 11.3 (0.5)
Final solvents [g/L] 13.2 (2.3) 16.9 (1.3) 16.4 (2.7) 15.1 (2.0) 16.7 (0.4) 19.8 (1.5) 20.4 (0.1)
Final solvent yield [g A/IBE /g glc. ] 0.36 (0.1) 0.27 (0.02) 0.29 (0.07) 0.29 (0.04) 0.30 (0.01) 0.29 (0.02) 0.30 (0.00)
Productivity after 30 h [g/L h] 0.37 (0.03) 0.38 (0.05) 0.34 (0.11) 0.26 (0.13) 0.31 (0.02) 0.54 (0.11) 0.67 (0.02)
Carbone recovery [%] 98% 90% 86% 101% 102% 96% 95%
  1. Cultures were performed in 1 L-working volume of CM1 containing 3.0 g/L ammonium acetate and 90 g/L glucose.
  2. All data are given as the mean of two or three fermentations the standard error to the mean is indicated in brackets.
  3. 1 a negative value (−) indicates that initial acetic acid from culture medium was partially consumed.
  4. 2 Fermentations were carried out at pH 5.0 but the pH set-point was not reached.