Table 3 Performance of C. acetobutylicum ATCC 824 and its transformants in 45‐h cultures performed with pH regulation at 5.0
Performances | C. beijerinckii 2 NRRL B5932 | C. acetobutylicum ATCC 824 | |||||
|---|---|---|---|---|---|---|---|
WT | (pMTL500E) | (pFC002) | (pFC005) | (pFC006) | (pFC007) | ||
Glucose consumed [g/L] | 36.8 (1.7) | 62.3 (0.4) | 62.5 (2.4) | 53.0 (7.9) | 56.3 (2.2) | 68.0 (4.3) | 68.1 (0.1) |
Acetic acid 1 [g/L] | −1.7 (0.0) | 0.3 (0.4) | 0.4 (1.8) | 1.8 (1.2) | 1.2 (0.2) | 1.0 (1.0) | 0.0 (0.1) |
Butyric acid [g/L] | 0.3 (0.1) | 1.7 (0.4) | 1.3 (0.4) | 2.6 (0.8) | 2.5 (0.5) | 2.1(1.6) | 1.4 (0.0) |
Acetoin [g/L] | 0.0 (0.0) | 1.1 (0.1) | 0.8 (0.1) | 0.0 (0.0) | 0.1 (0.0) | 0.0 (0.0) | 0.1 (0.0) |
2,3-Butanediol ( D or L ) [g/L] | 0.0 (0.0) | 0.0 (0.0) | 0.0 (0.0) | 0.5 (0.3) | 0.5 (0.1) | 0.6 (0.3) | 0.6 (0.1) |
Ethanol [g/L] | 0.1 (0.0) | 1.1 (0.2) | 0.8 (0.2) | 1.1 (0.3) | 0.7 (0.0) | 1.0 (0.3) | 0.8 (0.7) |
Acetone [g/L] | 0.2 (0.0) | 5.7 (0.0) | 5.4 (1.0) | 0.1 (0.1) | 0.1 (0.0) | 0.3 (0.2) | 0.9 (0.9) |
Isopropanol [g/L] | 4.5 (0.3) | 0.1 (0.1) | 0.1 (0.1) | 4.8 (0.8) | 6.1 (0.1) | 7.2 (0.5) | 7.3 (0.3) |
Butanol [g/L] | 8.4 (1.9) | 10.0 (1.5) | 10.1 (1.5) | 9.1 (1.6) | 9.7 (0.3) | 11.3 (0.8) | 11.3 (0.5) |
Final solvents [g/L] | 13.2 (2.3) | 16.9 (1.3) | 16.4 (2.7) | 15.1 (2.0) | 16.7 (0.4) | 19.8 (1.5) | 20.4 (0.1) |
Final solvent yield [g A/IBE /g glc. ] | 0.36 (0.1) | 0.27 (0.02) | 0.29 (0.07) | 0.29 (0.04) | 0.30 (0.01) | 0.29 (0.02) | 0.30 (0.00) |
Productivity after 30 h [g/L h] | 0.37 (0.03) | 0.38 (0.05) | 0.34 (0.11) | 0.26 (0.13) | 0.31 (0.02) | 0.54 (0.11) | 0.67 (0.02) |
Carbone recovery [%] | 98% | 90% | 86% | 101% | 102% | 96% | 95% |