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AMB Express

Table 4 Effect of different 4HB-related carbon sources concentrations on the biosynthesis of PHA by transformant  Cupriavidus necator  PHB¯4 harboring the PHA synthase gene of  Burkholderia  sp.

From: Revelation of the ability of Burkholderi a sp. USM (JCM 15050) PHA synthase to polymerize 4-hydroxybutyrate monomer

Carbon sourcesa

Carbon concentration (M)

Dry Cell Weightb(g/L)

PHA contentc(wt %)

PHA compositiond(mol %)

 

3HB

4HB

γ-butyrolactone

0.1

1.9 ± 0.1

10 ± 1

78

22

γ-butyrolactone

0.2

2.3 ± 0.1

29 ± 1

69

31

γ-butyrolactone

0.3

2.4 ± 0.2

31 ± 3

68

32

γ-butyrolactone

0.4

2.2 ± 0.1

24 ± 1

59

41

sodium 4-hydroxybutyrate

0.1

1.5 ± 0.1

Trace

28

72

sodium 4-hydroxybutyrate

0.2

2.0 ± 0.1

14 ± 2

13

87

sodium 4-hydroxybutyrate

0.3

1.9 ± 0.1

8 ± 1

40

60

sodium 4-hydroxybutyrate

0.4

2.0 ± 0.1

11 ± 2

46

54

  1. 3HB, 3-hydroxybutyrate; 4HB, 4-hydroxybutyrate.
  2. aCells were cultivated for 48 h, at 30 °C, 200 rpm in MM medium containing different concentrations of 4HB-related carbon sources at two-stage cultivation.
  3. bDry cell weight after freeze-drying.
  4. cPHA content of the freeze-dried cells was determined by gas chromatography.
  5. dPHA composition of the freeze-dried cells was determined by gas chromatography.
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