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Table 4 Effect of different 4HB-related carbon sources concentrations on the biosynthesis of PHA by transformant  Cupriavidus necator  PHB¯4 harboring the PHA synthase gene of  Burkholderia  sp.

From: Revelation of the ability of Burkholderi a sp. USM (JCM 15050) PHA synthase to polymerize 4-hydroxybutyrate monomer

Carbon sourcesa Carbon concentration (M) Dry Cell Weightb(g/L) PHA contentc(wt %) PHA compositiond(mol %)  
3HB 4HB
γ-butyrolactone 0.1 1.9 ± 0.1 10 ± 1 78 22
γ-butyrolactone 0.2 2.3 ± 0.1 29 ± 1 69 31
γ-butyrolactone 0.3 2.4 ± 0.2 31 ± 3 68 32
γ-butyrolactone 0.4 2.2 ± 0.1 24 ± 1 59 41
sodium 4-hydroxybutyrate 0.1 1.5 ± 0.1 Trace 28 72
sodium 4-hydroxybutyrate 0.2 2.0 ± 0.1 14 ± 2 13 87
sodium 4-hydroxybutyrate 0.3 1.9 ± 0.1 8 ± 1 40 60
sodium 4-hydroxybutyrate 0.4 2.0 ± 0.1 11 ± 2 46 54
  1. 3HB, 3-hydroxybutyrate; 4HB, 4-hydroxybutyrate.
  2. aCells were cultivated for 48 h, at 30 °C, 200 rpm in MM medium containing different concentrations of 4HB-related carbon sources at two-stage cultivation.
  3. bDry cell weight after freeze-drying.
  4. cPHA content of the freeze-dried cells was determined by gas chromatography.
  5. dPHA composition of the freeze-dried cells was determined by gas chromatography.