Skip to main content

Table 3 Effect of different carbon sources on the biosynthesis of PHA by transformant  Cupriavidus necator  PHB¯4 harboring the PHA synthase gene of  Burkholderia  sp.

From: Revelation of the ability of Burkholderi a sp. USM (JCM 15050) PHA synthase to polymerize 4-hydroxybutyrate monomer

Carbon sourcesa

Dry Cell Weightb(g/L)

PHA contentc(wt %)

PHA compositiond(mol %)

3HB

3HV

3H4MV

4HB

One-stage cultivation

      

pBBR1MCS-2  phaC   Bs  BE

      

CPKO

2.1 ± 0.4

64 ± 9

100

0

0

0

pBBR1MCS-2  phaC   Bs  BH

      

CPKO

2.4 ± 0.1

66 ± 2

100

0

0

0

Jatropha oil

2.3 ± 0.1

53 ± 1

100

0

0

0

Fructose

2.5 ± 0.1

61 ± 1

100

0

0

0

Fructose+ 4MV

1.6 ± 0.2

40 ± 8

97

0

3

0

Two-stage cultivation

      

Sodium propionate

2.7 ± 0.1

27 ± 3

70

30

0

0

Sodium valerate

3.2 ± 0.1

35 ± 1

60

40

0

0

γ-butyrolactone

2.3 ± 0.1

29 ± 1

69

0

0

31

sodium 4-hydroxybutyrate

2.0 ± 0.1

14 ± 2

13

0

0

87

  1. CPKO, crude palm kernel oil; 4MV, 4-methylvaleric acid; 3HB, 3-hydroxybutyrate; 3HV, 3-hydroxyvalerate 3H4MV, 3-hydroxy-4-methylvalerate; 4HB, 4-hydroxybutyrate.
  2. aCells were cultivated for 48 h, at 30 °C, 200 rpm in MM medium containing the indicated carbon sources (0.2 M carbon concentration: CPKO, jatropha oil, fructose, sodium propionate, sodium valerate, γ-butyrolactone, sodium 4-hydroxybutyrate and 0.02 M carbon concentration: 4-methylvaleric acid).
  3. bDry cell weight after freeze-drying.
  4. dPHA composition of the freeze-dried cells was determined by gas chromatography.