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Table 3 Effect of different carbon sources on the biosynthesis of PHA by transformant  Cupriavidus necator  PHB¯4 harboring the PHA synthase gene of  Burkholderia  sp.

From: Revelation of the ability of Burkholderi a sp. USM (JCM 15050) PHA synthase to polymerize 4-hydroxybutyrate monomer

Carbon sourcesa Dry Cell Weightb(g/L) PHA contentc(wt %) PHA compositiond(mol %)
3HB 3HV 3H4MV 4HB
One-stage cultivation       
pBBR1MCS-2  phaC   Bs  BE       
CPKO 2.1 ± 0.4 64 ± 9 100 0 0 0
pBBR1MCS-2  phaC   Bs  BH       
CPKO 2.4 ± 0.1 66 ± 2 100 0 0 0
Jatropha oil 2.3 ± 0.1 53 ± 1 100 0 0 0
Fructose 2.5 ± 0.1 61 ± 1 100 0 0 0
Fructose+ 4MV 1.6 ± 0.2 40 ± 8 97 0 3 0
Two-stage cultivation       
Sodium propionate 2.7 ± 0.1 27 ± 3 70 30 0 0
Sodium valerate 3.2 ± 0.1 35 ± 1 60 40 0 0
γ-butyrolactone 2.3 ± 0.1 29 ± 1 69 0 0 31
sodium 4-hydroxybutyrate 2.0 ± 0.1 14 ± 2 13 0 0 87
  1. CPKO, crude palm kernel oil; 4MV, 4-methylvaleric acid; 3HB, 3-hydroxybutyrate; 3HV, 3-hydroxyvalerate 3H4MV, 3-hydroxy-4-methylvalerate; 4HB, 4-hydroxybutyrate.
  2. aCells were cultivated for 48 h, at 30 °C, 200 rpm in MM medium containing the indicated carbon sources (0.2 M carbon concentration: CPKO, jatropha oil, fructose, sodium propionate, sodium valerate, γ-butyrolactone, sodium 4-hydroxybutyrate and 0.02 M carbon concentration: 4-methylvaleric acid).
  3. bDry cell weight after freeze-drying.
  4. dPHA composition of the freeze-dried cells was determined by gas chromatography.