|
Strains
| | |
|
E. coli JM109
|
recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac-proAB)/F'[traD36, proAB+, lacIq, lacZ ΔM15]
|
Promega
|
|
E. coli S17−1
|
relA1, ΔlacU169, (ϕ80lacZ ΔM15), thi1, proA, hsdR17, hsdM+, recA, RP4-tra function
|
Simon et al., [1983]
|
|
C. necator PHB¯4
|
PHA-negative mutant of wild-type H16
|
Schlegel et al., [1970]
|
|
Burkholderia sp. USM (JCM 15050)
|
Wild type
|
Chee et al., [2010]
|
|
Plasmids
| | |
|
pGEM-T
|
Apr, lacZ, cloning vector
|
Promega
|
|
pBBR1MCS-2
|
Kmr, lacPOZ' mob+, broad host range
|
Kovach et al., [1995]
|
|
pBBR1MCS-2 phaC
Bs
BH
|
pBBR1MCS-2 derivative harbouring Bam HI-Hind III phaC from Burkholderia sp. with putative promoter
|
This study
|
|
pBBR1MCS-2 phaC
Bs
BE
|
pBBR1MCS-2 derivative harbouring Bam HI-Eco RI phaC from Burkholderia sp. with putative promoter
|
This study
|
