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Table 1 Bacterial strains and plasmids used in this study

From: Revelation of the ability of Burkholderi a sp. USM (JCM 15050) PHA synthase to polymerize 4-hydroxybutyrate monomer

Strain or plasmid Relevant characteristics Source or reference
Strains   
E. coli JM109 recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac-proAB)/F'[traD36, proAB+, lacIq, lacZ ΔM15] Promega
E. coli S17−1 relA1, ΔlacU169, (ϕ80lacZ ΔM15), thi1, proA, hsdR17, hsdM+, recA, RP4-tra function Simon et al., [1983]
C. necator PHB¯4 PHA-negative mutant of wild-type H16 Schlegel et al., [1970]
Burkholderia sp. USM (JCM 15050) Wild type Chee et al., [2010]
Plasmids   
pGEM-T Apr, lacZ, cloning vector Promega
pBBR1MCS-2 Kmr, lacPOZ' mob+, broad host range Kovach et al., [1995]
pBBR1MCS-2 phaC Bs BH pBBR1MCS-2 derivative harbouring Bam HI-Hind III phaC from Burkholderia sp. with putative promoter This study
pBBR1MCS-2 phaC Bs BE pBBR1MCS-2 derivative harbouring Bam HI-Eco RI phaC from Burkholderia sp. with putative promoter This study