Table 1 Bacterial strains and plasmids used in this study

Strains

E. coli JM109

recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac-proAB)/F'[traD36, proAB⁺, lacI^q, lacZ ΔM15]

Promega

E. coli S17−1

relA1, ΔlacU169, (ϕ80lacZ ΔM15), thi1, proA, hsdR17, hsdM⁺, recA, RP4-tra function

Simon et al., [1983]

C. necator PHB¯4

PHA-negative mutant of wild-type H16

Schlegel et al., [1970]

Burkholderia sp. USM (JCM 15050)

Wild type

Chee et al., [2010]

Plasmids

pGEM-T

Ap^r, lacZ, cloning vector

Promega

pBBR1MCS-2

Km^r, lacPOZ' mob⁺, broad host range

Kovach et al., [1995]

pBBR1MCS-2 phaC _Bs BH

pBBR1MCS-2 derivative harbouring Bam HI-Hind III phaC from Burkholderia sp. with putative promoter

This study

pBBR1MCS-2 phaC _Bs BE

pBBR1MCS-2 derivative harbouring Bam HI-Eco RI phaC from Burkholderia sp. with putative promoter

This study