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Table 1 Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli JM109 | recA1, endA1, gyrA96, thi, hsdR17, supE44, relA1, Δ(lac-proAB)/F'[traD36, proAB+, lacIq, lacZ ΔM15] | Promega |
| E. coli S17−1 | relA1, ΔlacU169, (ϕ80lacZ ΔM15), thi1, proA, hsdR17, hsdM+, recA, RP4-tra function | Simon et al., [1983] |
| C. necator PHB¯4 | PHA-negative mutant of wild-type H16 | Schlegel et al., [1970] |
| Burkholderia sp. USM (JCM 15050) | Wild type | Chee et al., [2010] |
| Plasmids | ||
| pGEM-T | Apr, lacZ, cloning vector | Promega |
| pBBR1MCS-2 | Kmr, lacPOZ' mob+, broad host range | Kovach et al., [1995] |
| pBBR1MCS-2 phaC Bs BH | pBBR1MCS-2 derivative harbouring Bam HI-Hind III phaC from Burkholderia sp. with putative promoter | This study |
| pBBR1MCS-2 phaC Bs BE | pBBR1MCS-2 derivative harbouring Bam HI-Eco RI phaC from Burkholderia sp. with putative promoter | This study |
