Skip to main content

Table 3 Effect of L-cysteine on the activities of recombinant wild-type and M57L mutant CgPAD

From: An endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from Candida guilliermondii

Substrate Specific activity (U mg-1) Relative activity b (%)
Wild type   
FA 260 ± 9.0 100
FA + L-cysteine a 759 ± 156 292
PCA 259 ± 4.0 100
PCA + L-cysteine 257 ± 3.3 99
CA 31.7 ± 2.8 12
CA + L-cysteine 35.9 ± 3.5 14
M57L   
FA 20.1 ± 0.3 100
FA + L-cysteine a 66.7 ± 8.5 332
PCA 20.5 ± 0.3 100
PCA + L-cysteine 26.0 ± 2.7 127
CA < 2.6 < 13
CA + L-cysteine ND c  
  1. Experimental conditions were the same as those described in Figure 3. a Treated with 1 mM. b The activity toward FA is taken as 100%. c Not determined.