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Table 3 Effect of L-cysteine on the activities of recombinant wild-type and M57L mutant CgPAD

From: An endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from Candida guilliermondii

Substrate

Specific activity (U mg-1)

Relative activity b (%)

Wild type

  

FA

260 ± 9.0

100

FA + L-cysteine a

759 ± 156

292

PCA

259 ± 4.0

100

PCA + L-cysteine

257 ± 3.3

99

CA

31.7 ± 2.8

12

CA + L-cysteine

35.9 ± 3.5

14

M57L

  

FA

20.1 ± 0.3

100

FA + L-cysteine a

66.7 ± 8.5

332

PCA

20.5 ± 0.3

100

PCA + L-cysteine

26.0 ± 2.7

127

CA

< 2.6

< 13

CA + L-cysteine

ND c

 
  1. Experimental conditions were the same as those described in Figure 3. a Treated with 1 mM. b The activity toward FA is taken as 100%. c Not determined.