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Table 2 Effects of thiol reagents on the activities of native CgPAD

From: An endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from Candida guilliermondii

Additive (1 mM)

Specific activity (U mg-1)

FA/PCA

 

FA (%)

PCA (%)

 

None

378 ± 16 (100)

393 ± 6 (100)

0.96

2-Mercaptoethanol (5 mM)

806 ± 56 (213)

440 ± 3 (112)

1.83

Dithiothreitol

1140 ± 177 (302)

598 ± 7 (112)

1.91

L-Cysteine

1130 ± 37 (299)

437 ± 39 (111)

2.59

D-Cysteine

1590 ± 252 (421)

590 ± 4 (150)

2.69

DL-Homocysteine

1830 ± 354 (484)

628 ± 9 (160)

2.91

Cysteic acid

557 ± 30 (147)

593 ± 9 (151)

0.94

  1. Experimental conditions were the same as those described in Figure 3.