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Table 2 Effects of thiol reagents on the activities of native CgPAD

From: An endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from Candida guilliermondii

Additive (1 mM) Specific activity (U mg-1) FA/PCA
  FA (%) PCA (%)  
None 378 ± 16 (100) 393 ± 6 (100) 0.96
2-Mercaptoethanol (5 mM) 806 ± 56 (213) 440 ± 3 (112) 1.83
Dithiothreitol 1140 ± 177 (302) 598 ± 7 (112) 1.91
L-Cysteine 1130 ± 37 (299) 437 ± 39 (111) 2.59
D-Cysteine 1590 ± 252 (421) 590 ± 4 (150) 2.69
DL-Homocysteine 1830 ± 354 (484) 628 ± 9 (160) 2.91
Cysteic acid 557 ± 30 (147) 593 ± 9 (151) 0.94
  1. Experimental conditions were the same as those described in Figure 3.