An endogenous factor enhances ferulic acid decarboxylation catalyzed by phenolic acid decarboxylase from Candida guilliermondii

AMB Express

Table 1 Substrate specificities of native and recombinant enzymes

Enzyme	Substrate	Specific activity (U mg^-1)	Relative activity ^a (%)
Native	FA	378 ± 16	100
	PCA	393 ± 6	104
	CA	53 ± 9	14
Recombinant	Wild-type
	FA	260 ± 30	100
	PCA	259 ± 4.0	100
	CA	31.7 ± 2.8	12
	M57L
	FA	20.1 ± 0.3	100
	PCA	20.5 ± 0.3	102
	CA	2.93 ± 0.03	15
	M57T
	FA	< 0.1	-
	PCA	< 0.1	-
	CA	< 0.01	-
	M57A
	FA	< 1.0	-
	PCA	< 1.0	-
	CA	< 0.02	-

^a The FA decarboxylation activities of the native and recombinant enzymes are taken as 100% for each.