PCR-amplification confirms the presence of both the FLO5 and EPG1 cassettes in the recombinant yeast strains. Lane 1: Wild type strain containing the EPG1 cassette (2772 bp). Lane 2: Inducible flocculent phenotype containing the FLO5 (4921 bp) and EPG1 cassettes. Lane 3: Constitutive flocculent phenotype displaying the FLO5 and EPG1 cassettes. Lane 4: Molecular Weight Marker (2-Log DNA Ladder, New England Biolabs). Lane 5: The wild type strain does not contain a FLO5 gene. Lane 6: The FLO5 gene was PCR-amplified (3227 bp) from the inducible flocculent phenotype Lane 7: The FLO5 gene amplified from the constitutive flocculent phenotype.