Figure 1

Construction of the K. marxianus FLO5 expression vector (pEPG-FLO5). A) The intermediate plasmid pEPG was designed by introducing an EPG1 cassette into the pGAPZα vector. B) The pEPG-FLO5 expression vector was constructed by replacing the EPG gene, in pEPG, with the FLO5 gene. The plasmid map denotes the position of the recognition sites for the restriction enzymes Sac I, used for plasmid linearization to achieve homologous recombination, and Ppi I, the enzyme used to linearize the DNA for random genomic insertion.