Novel amidases of two Aminobacter sp. strains: Biotransformation experiments and elucidation of gene sequences

AMB Express

Table 1 Location and properties of genes and deduced proteins of the sequenced fragments of Aminobacter sp. DSM24754 and Aminobacter sp. DSM24755 respectively

*Aminobacter* sp.	Gene	Nucleotide position	GC (%)	Putative RBS	Protein length (aa)	Calculated molecular mass (kDa)	Protein with highest identity in BLAST	Accession number	Organism	Identical amino acids (%)
DSM24754	ORF1	274-1215	62	TGAGGAGG TAAGACCATG	313	35	N-carbamoyl-D-amino acid amidohydrolase	BAD00008.1	Pseudomonas sp. KNK003A	85
	ORF2	(1238)/	(62.5)/	ATG ACGGCAATCAATGGGAGA ACGAGCATG	(494)/	(53.8)/	putative dihydropyrimidinase	YP_675206	Mesorhizobium sp. BNC1	83
	ORF2	1265-2722	62.7	ATG ACGGCAATCAATGGGAGA ACGAGCATG	485	52.9	putative dihydropyrimidinase	YP_675206	Mesorhizobium sp. BNC1	83
	ORF3	3252-2818	59.8	-	-	-	hypothetical protein	YP_002542208	Agrobacterium radiobacter K84	78
DSM24755	ORF1	80-1531	63.6	TAGGGG AACTGAAGAAATG	483	53.2	putative phenylhydantoinase	NP_103173.1	Mesorhizobium loti MAFF303099	90
DSM24755	ORF2	1780-2601	62.8	TGGGG TAAAACCGGGCAATG	273	67.5	putative ATP-binding protein of ABC transporter	NP_103169.1	Mesorhizobium loti MAFF303099	91
	ORF3	2607-3127	62.8	-	-	-	putative permease protein of ABC transporter	ZP_09089625.1	Mesorhizobium amorphae CCNWGS0123	88

(−) no data available, due to an incomplete open reading frame. GC = guanine-cytosine content; aa = amino acids, (*) = values in brackets are for the 1485 bp long gene fragment of ORF2; underlined sequence parts = putative Shine-Dalgarno sequence (RBS), sequence in boldface = assumed start codon, sequence in italics = potential alternative start codon.