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Table 4 Effects of various compounds on  S  -HMGSH dehydrogenase activity

From: Purification and properties of S-hydroxymethylglutathione dehydrogenase of Paecilomyces variotii no. 5, a formaldehyde-degrading fungus

Compound

Concentration

Remaining

 

(mM)

activity (%)

None

 

100

NiCl2

1

0

MnCl2

1

179

CoSO4

1

40

FeCl2

1

95

FeCl3

1

0

BaCl2

1

132

CaCl2

1

112

HgCl2

1

0

MgCl2

1

49

NaCl

1

84

EDTA

10

92

PCMB

1

0

Ellman reagent

1

68

PMSF

1

11

Cuprizone

1

0

Hydroxyl amine

1

60

Semicarbazide

1

67

Dithiothreitol

1

92

  1. The enzyme in Tris–HCl buffer (pH 8.0) was treated by various compounds at 30°C for 1 hr and the reaction was carried out at pH 8.0 for 10 min.
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