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Table 4 Effects of various compounds on  S  -HMGSH dehydrogenase activity

From: Purification and properties of S-hydroxymethylglutathione dehydrogenase of Paecilomyces variotii no. 5, a formaldehyde-degrading fungus

Compound Concentration Remaining
  (mM) activity (%)
None   100
NiCl2 1 0
MnCl2 1 179
CoSO4 1 40
FeCl2 1 95
FeCl3 1 0
BaCl2 1 132
CaCl2 1 112
HgCl2 1 0
MgCl2 1 49
NaCl 1 84
EDTA 10 92
PCMB 1 0
Ellman reagent 1 68
PMSF 1 11
Cuprizone 1 0
Hydroxyl amine 1 60
Semicarbazide 1 67
Dithiothreitol 1 92
  1. The enzyme in Tris–HCl buffer (pH 8.0) was treated by various compounds at 30°C for 1 hr and the reaction was carried out at pH 8.0 for 10 min.