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Table 2 Summary of purification of  S  -HMGSH dehydrogenase from  P. variotii  NBRC 109023

From: Purification and properties of S-hydroxymethylglutathione dehydrogenase of Paecilomyces variotii no. 5, a formaldehyde-degrading fungus

 

Total protein

Total activity

Specific activity

Yield

Purification

 

(mg)

(Units)

(U/mg)

(%)

(fold)

Cell-free extract

10,820

23,312

2.2

100

1

Ammonium sulfate

1,539

11,080

7.2

47.5

3.3

DEAE-Sepharose

113

7,912

70

33.9

32

Hydroxyapatite

13.1

5,079

388

21.8

176

Lyophilization

10.0

2,503

250

10.7

114

Isoelectrofocusing

9.1

2,452

269

10.5

122