Total protein | Total activity | Specific activity | Yield | Purification | |
---|---|---|---|---|---|
(mg) | (Units) | (U/mg) | (%) | (fold) | |
Cell-free extract | 10,820 | 23,312 | 2.2 | 100 | 1 |
Ammonium sulfate | 1,539 | 11,080 | 7.2 | 47.5 | 3.3 |
DEAE-Sepharose | 113 | 7,912 | 70 | 33.9 | 32 |
Hydroxyapatite | 13.1 | 5,079 | 388 | 21.8 | 176 |
Lyophilization | 10.0 | 2,503 | 250 | 10.7 | 114 |
Isoelectrofocusing | 9.1 | 2,452 | 269 | 10.5 | 122 |