Skip to main content

Table 2 Summary of purification of  S  -HMGSH dehydrogenase from  P. variotii  NBRC 109023

From: Purification and properties of S-hydroxymethylglutathione dehydrogenase of Paecilomyces variotii no. 5, a formaldehyde-degrading fungus

  Total protein Total activity Specific activity Yield Purification
  (mg) (Units) (U/mg) (%) (fold)
Cell-free extract 10,820 23,312 2.2 100 1
Ammonium sulfate 1,539 11,080 7.2 47.5 3.3
DEAE-Sepharose 113 7,912 70 33.9 32
Hydroxyapatite 13.1 5,079 388 21.8 176
Lyophilization 10.0 2,503 250 10.7 114
Isoelectrofocusing 9.1 2,452 269 10.5 122