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Table 1 Bacterial strains and plasmids used in this study

From: Branching of the p-nitrophenol (PNP) degradation pathway in burkholderia sp. Strain SJ98: Evidences from genetic characterization of PNP gene cluster

Bacterial strain/plasmid/cosmid

Characteristic

Source or reference

Bacterial strains

  

Burkholderia sp. strain SJ98

Wild type PNP degrading isolate

Lab stock and DSM = 23195

E. coli DH5α

Host strain for cosmid cloning vectorHost strain for GateWay entry clone

Lab stock

E. coli BL-21 AI

Host strain for expression vector (LacY1DE3, F_ompT hsdS, gal, dcm, ara-I)

Invitrogen Inc. CA- USA

Plasmid/cosmid

  

SuperCos-1

Cosmid cloning vector, Dual cos sites

Agilent Technologies- Genomics. CA, USA.

pSJC88

Cosmid clone with ~41 Kb insert harboring PNP degrading gene cluster of strain SJ98

This study

pDONR-201

Gateway entry cloning vector

Invitrogen Inc. CA, USA

pDEST-17

Gateway expression vector

Invitrogen Inc. CA, USA

pDest-pnpE1

Expression clone with orf pnpE1 of strain SJ98

This study

pDest-pnpE2

Expression clone with orf pnpE2 of strain SJ98

This study