Bacterial strains
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Burkholderia sp. strain SJ98
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Wild type PNP degrading isolate
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Lab stock and DSM = 23195
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E. coli DH5α
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Host strain for cosmid cloning vectorHost strain for GateWay entry clone
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Lab stock
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E. coli BL-21 AI
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Host strain for expression vector (LacY1DE3, F_ompT hsdS, gal, dcm, ara-I)
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Invitrogen Inc. CA- USA
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Plasmid/cosmid
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SuperCos-1
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Cosmid cloning vector, Dual cos sites
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Agilent Technologies- Genomics. CA, USA.
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pSJC88
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Cosmid clone with ~41 Kb insert harboring PNP degrading gene cluster of strain SJ98
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This study
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pDONR-201
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Gateway entry cloning vector
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Invitrogen Inc. CA, USA
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pDEST-17
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Gateway expression vector
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Invitrogen Inc. CA, USA
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pDest-pnpE1
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Expression clone with orf pnpE1 of strain SJ98
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This study
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pDest-pnpE2
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Expression clone with orf pnpE2 of strain SJ98
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This study
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