Table 1 Bacterial strains and plasmids used in this study
Bacterial strain/plasmid/cosmid | Characteristic | Source or reference |
|---|---|---|
Bacterial strains | ||
Burkholderia sp. strain SJ98 | Wild type PNP degrading isolate | Lab stock and DSM = 23195 |
E. coli DH5α | Host strain for cosmid cloning vectorHost strain for GateWay entry clone | Lab stock |
E. coli BL-21 AI | Host strain for expression vector (LacY1DE3, F_ompT hsdS, gal, dcm, ara-I) | Invitrogen Inc. CA- USA |
Plasmid/cosmid | ||
SuperCos-1 | Cosmid cloning vector, Dual cos sites | Agilent Technologies- Genomics. CA, USA. |
pSJC88 | Cosmid clone with ~41 Kb insert harboring PNP degrading gene cluster of strain SJ98 | This study |
pDONR-201 | Gateway entry cloning vector | Invitrogen Inc. CA, USA |
pDEST-17 | Gateway expression vector | Invitrogen Inc. CA, USA |
pDest-pnpE1 | Expression clone with orf pnpE1 of strain SJ98 | This study |
pDest-pnpE2 | Expression clone with orf pnpE2 of strain SJ98 | This study |