|
Bacterial strains
| | |
|
Burkholderia sp. strain SJ98
|
Wild type PNP degrading isolate
|
Lab stock and DSM = 23195
|
|
E. coli DH5α
|
Host strain for cosmid cloning vectorHost strain for GateWay entry clone
|
Lab stock
|
|
E. coli BL-21 AI
|
Host strain for expression vector (LacY1DE3, F_ompT hsdS, gal, dcm, ara-I)
|
Invitrogen Inc. CA- USA
|
|
Plasmid/cosmid
| | |
|
SuperCos-1
|
Cosmid cloning vector, Dual cos sites
|
Agilent Technologies- Genomics. CA, USA.
|
|
pSJC88
|
Cosmid clone with ~41 Kb insert harboring PNP degrading gene cluster of strain SJ98
|
This study
|
|
pDONR-201
|
Gateway entry cloning vector
|
Invitrogen Inc. CA, USA
|
|
pDEST-17
|
Gateway expression vector
|
Invitrogen Inc. CA, USA
|
|
pDest-pnpE1
|
Expression clone with orf pnpE1 of strain SJ98
|
This study
|
|
pDest-pnpE2
|
Expression clone with orf pnpE2 of strain SJ98
|
This study
|
