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Figure 3 | AMB Express

Figure 3

From: Branching of the p-nitrophenol (PNP) degradation pathway in burkholderia sp. Strain SJ98: Evidences from genetic characterization of PNP gene cluster

Figure 3

(A) Enzyme activity of hydroquinone dioxygenase assayed by UV-Visible spectrophotometer. (i) Negative control for the hydroquinone transformation E. coli BL21-AI (without pDEST-pnpE1 and pDEST-pnpE2) cell lysate (ii) Hydroquinone transformed into γ-hydroxymuconic semialdehyde and detected at the wavelength 320 nm. (B) The percent activity of the hydroquinone at different (i) pHs, (ii) Temperatures and (iii) The Michaelis-menten curve for the hydroquinone dioxygenase activity.

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