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AMB Express
Figure 2 | AMB Express

Figure 2

From: Branching of the p-nitrophenol (PNP) degradation pathway in burkholderia sp. Strain SJ98: Evidences from genetic characterization of PNP gene cluster

Figure 2

(A) SDS PAGE gel showing the heterologous expression of PnpE1 and PnpE2 in E. coli BL21 AI. The lane M: Marker; Lane 1 & 4: Uninduced supernatant of pnpE1 and pnpE2; Lane 2 & 5: Induced whole cell lysate of pnpE1 and pnpE2; Lane 3 & 6: Supernatant of pnpE1 and pnpE2 (pnpE1 not present in the supernatant); Lane 7 & 8: purified and pnpE1 (refolded) and pnpE2 respectively (B) Size exclusion chromatography of subunits of hydroquinone dioxygenase using sephacryl-200 (i) Gel filtration of PnpE1 eluted at 59.1 ml from the column and found to be a monomer of 40 kDa, (ii) Gel filtration of PnpE2 eluted at 65 ml and found to be a monomer of 20 kDa, (iii) Mixture of both the subunits found to be eluted at approximately 44 ml and the molecular weight of the hydroquinone dioxygenase predicted as approximately 120 kDa.

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