AMB Express

Table 1 Kinetic parameters of LeHex20A

From: Characterization of β-N-acetylhexosaminidase (LeHex20A), a member of glycoside hydrolase family 20, from Lentinula edodes (shiitake mushroom)

Substrate	K_m(mM)	k_cat(s-1)	k_cat/K_m
pNP-GlcNAc	0.34 ± 0.01	335 ± 10	983
pNP-GalNAc	0.43 ± 0.03	177 ± 8	411
Chitobiose(DP2)	0.42 ± 0.04	242 ± 14	576.7
Chitotriose(DP3)	0.14 ± 0.001	236 ± 1	1667
Chitotetraose(DP4)	0.07 ± 0.008	119 ± 5	1689
Chitopentaose(DP5)	0.08 ± 0.001	97 ± 1	1190
Chitohexaose(DP6)	0.1 ± 0.002	97 ± 1	992

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