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Table 1 Kinetic parameters of LeHex20A

From: Characterization of β-N-acetylhexosaminidase (LeHex20A), a member of glycoside hydrolase family 20, from Lentinula edodes (shiitake mushroom)

Substrate Km(mM) kcat(s-1) kcat/Km
pNP-GlcNAc 0.34 ± 0.01 335 ± 10 983
pNP-GalNAc 0.43 ± 0.03 177 ± 8 411
Chitobiose(DP2) 0.42 ± 0.04 242 ± 14 576.7
Chitotriose(DP3) 0.14 ± 0.001 236 ± 1 1667
Chitotetraose(DP4) 0.07 ± 0.008 119 ± 5 1689
Chitopentaose(DP5) 0.08 ± 0.001 97 ± 1 1190
Chitohexaose(DP6) 0.1 ± 0.002 97 ± 1 992