HPLC analysis of LeHex20A action on colloidal chitin. Colloidal chitin (2%, w/v) was incubated with LeHex20A (0.4 nM) in 20 mM sodium acetate buffer (pH 4.2), at 30°C. Eluted products were detected by monitoring UV absorption at 205 nm. Standards (Std.) were GlcNAc and chitooligosaccharides (GlcNAc2-6). DP; degree of polymerization.