Schematic plasmid map of pKOV410 -mCherry-YlCWP1 expression vector and of the cell wall fusion proteins. ( A) mCherry-YlCWP1 fusion gene in plasmid pKOV410- mCherry-YlCWP1 under the transcriptional control of hp4d promoter, pre-pro Lip2 for secretion of fusion proteins and terminator (Term). The plasmid contains URA3d4 and KanR markers for selection. ( B) mCherry-YlCWP1 covalently binds to β-1,6-glucan in the cell wall of Y. lipolytica through glycosylphosphatidylinositol (GPI) anchor. The plasmid map was constructed using Geneious v5.5 ([Drummond et al. 2011]).