Skip to main content
AMB Express

Table 4 Concentration of phaZ1 - phaZ5 cDNA [ng/μL] detected in RT-qPCR for H16/pBBR1MCS-2, H16/pER1, H16/pER2, H16/pER3, H16/pER4 amplified from 100 ng of RNA

From: Examination of PHB Depolymerases in Ralstonia eutropha: Further Elucidation of the Roles of Enzymes in PHB Homeostasis

Genea

Plasmid presentb

Concentration of cDNA [ng/μL]c

phaZ1

pER1

4.21E-06

3.05E-06

phaZ1

pBBR1MCS-2

3.07E-07

2.63E-07

phaZ2

pER2

1.22E-04

6.45E-05

phaZ2

pBBR1MCS-2

ndd

ndd

phaZ3

pER3

3.68E-07

1.01E-07

phaZ3

pBBR1MCS-2

ndd

ndd

phaZ5

pER4

3.07E-07

6.35E-07

phaZ5

pBBR1MCS-2

2.92E-09

  

8.48E-10

  1. aIndicates the primer pair of the corresponding phaZ gene used in the RT-qPCR reaction.
  2. bIndicates the overexpression plasmid, or empty vector (pBBR1MCS-2), present in the R. eutropha H16 host strain.
  3. cTop and bottom numbers of each sample represent results of two different experiments (ex. „4.21E-06“ corresponds to 4.21 × 10-6).
  4. dTranscript not detected by RT-qPCR.
Back to article page