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Table 4 Concentration of phaZ1 - phaZ5 cDNA [ng/μL] detected in RT-qPCR for H16/pBBR1MCS-2, H16/pER1, H16/pER2, H16/pER3, H16/pER4 amplified from 100 ng of RNA

From: Examination of PHB Depolymerases in Ralstonia eutropha: Further Elucidation of the Roles of Enzymes in PHB Homeostasis

Genea Plasmid presentb Concentration of cDNA [ng/μL]c
phaZ1 pER1 4.21E-06
3.05E-06
phaZ1 pBBR1MCS-2 3.07E-07
2.63E-07
phaZ2 pER2 1.22E-04
6.45E-05
phaZ2 pBBR1MCS-2 ndd
ndd
phaZ3 pER3 3.68E-07
1.01E-07
phaZ3 pBBR1MCS-2 ndd
ndd
phaZ5 pER4 3.07E-07
6.35E-07
phaZ5 pBBR1MCS-2 2.92E-09
   8.48E-10
  1. aIndicates the primer pair of the corresponding phaZ gene used in the RT-qPCR reaction.
  2. bIndicates the overexpression plasmid, or empty vector (pBBR1MCS-2), present in the R. eutropha H16 host strain.
  3. cTop and bottom numbers of each sample represent results of two different experiments (ex. „4.21E-06“ corresponds to 4.21 × 10-6).
  4. dTranscript not detected by RT-qPCR.