Table 3 Primers used in this study
Name/purpose | Sequence(5’-3’)* |
|---|---|
Deletion of phaZ3 gene | |
phaZ3del1 | CAT AGA GCT C AT CGC TGC GGC AAC TTG GG |
phaZ3del2 | GAC ACT GCA G CA TGA AGA CTC CCG TAG GAA |
phaZ3del3 | GAT ACT GCA G AC TTA GCA TCG CCT GCC C |
phaZ3del4 | CAA GTC TAG A GA CGC GTG AAC AAG CTG |
phaZ3delchkFW | CGG TGA ACC ATC GAA TTC |
phaZ3delchkRV | CAA GGT TGA CCG CAG CAA G |
Plasmid-based overexpression of phaZ genes | |
PhaZ1 FW | GCA CGG TAC C AT GCT CTA CCA ATT GCA TGA G |
Pha Z1 RV | GAT ATC TAG A TT ACC TGG TGG CCG AGG CCT G |
Pha Z2 FW | CAT AGG TAC C AT GCT GTA CCA CGC CTA CCA |
Pha Z2 RV | GCG TTC TAG A TT AGC TGC TGG TGT AGA TGG |
Pha Z3 FW | CTA TGT CGA C AT GCT GTA CCA GCT CGT CGA G |
Pha Z3 RW | CAA GTC TAG A CT AAG TAT CGT GCC CGG TCA |
Pha Z5 FW | AAT TGG TAC C AT GGC GCT CTA TGC TCT GCG |
Pha Z5 RW | GAA TTC TAG A TC AGT CGG CGC ACC GTT GAA |
RT-PCR to quantify overexpression of phaZ genes | |
PhaZ1 FW | CCATCAAGCTGCTCAAGGAT |
Pha Z1 RV | CCAGTCGGTGACGTAGACCT |
Pha Z2 FW | TCTACCTGGAAACCGTCAGC |
Pha Z2 RV | CAGATATCGTCGCGTTCACC |
Pha Z3 FW | GGACTATTGCCTGGATCTCG |
Pha Z3 RV | AATTCCTGGAACACGAGCTG |
Pha Z5 FW | ATGATCGAGGCAGGTTATCG |
Pha Z5 RV | GACCTCGTCGATCTCAAAGC |