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Table 2 Bacterial strains and plasmids used in this study

From: Examination of PHB Depolymerases in Ralstonia eutropha: Further Elucidation of the Roles of Enzymes in PHB Homeostasis

Strain or Plasmid Description Reference
R. eutropha strains
H16 Wild type, Gm resistant ATCC 17699
Re1097 H16/ΔphaZ1 ([York et al. 2003])
Re1107 H16/ΔphaZ5 ([York et al. 2003])
Re1110 H16/ΔphaZ2 ([York et al. 2003])
Re1111 H16/ΔphaZ1 ΔphaZ ΔphaZ5 ([York et al. 2003])
Re1112 H16/ΔphaZ1 ΔphaZ2 ([York et al. 2003])
Re2005 H16/ΔphaZ1 ΔphaZ2 ΔphaZ3 ΔphaZ5 This study
Re2006 H16/ΔphaZ3 This study
E. coli strains
Top10 IF-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZ ΔM15 ΔlacX 74 recA 1 araD 139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA 1 nupG λ- Invitrogen
S17-1 recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 ATCC47055; ([Simon et al. 1983])
Plasmids
pBBR1MCS-2 Broad host range cloning vector, confers Km resistance ([Kovach et al. 1995])
pER1 pBBR1MCS-2 with phaZ1 inserted into Kpn1/Xba1 restriction sites, confers Km resistance This study
pER2 pBBR1MCS-2 with phaZ2 inserted into Kpn1/Xba1 restriction sites, confers Km resistance This study
pER3 pBBR1MCS-2 with phaZ3 inserted into Sal1/Xba1 restriction sites, confers Km resistance This study
pER4 pBBR1MCS-2 with phaZ5 inserted into Kpn1/Xba1 restriction sites, confers Km resistance This study
pGY46 phaC precise-deletion gene replacement plasmid, confers Km resistance ([York et al. 2001])
pCJB3 pGY46 with regions upstream and downstram of phaZ3 inserted between SacI and XbaI This study