Table 2 Bacterial strains and plasmids used in this study
Strain or Plasmid | Description | Reference |
|---|---|---|
R. eutropha strains | ||
H16 | Wild type, Gm resistant | ATCC 17699 |
Re1097 | H16/ΔphaZ1 | ([York et al. 2003]) |
Re1107 | H16/ΔphaZ5 | ([York et al. 2003]) |
Re1110 | H16/ΔphaZ2 | ([York et al. 2003]) |
Re1111 | H16/ΔphaZ1 ΔphaZ ΔphaZ5 | ([York et al. 2003]) |
Re1112 | H16/ΔphaZ1 ΔphaZ2 | ([York et al. 2003]) |
Re2005 | H16/ΔphaZ1 ΔphaZ2 ΔphaZ3 ΔphaZ5 | This study |
Re2006 | H16/ΔphaZ3 | This study |
E. coli strains | ||
Top10 | IF-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZ ΔM15 ΔlacX 74 recA 1 araD 139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA 1 nupG λ- | Invitrogen |
S17-1 | recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 | ATCC47055; ([Simon et al. 1983]) |
Plasmids | ||
pBBR1MCS-2 | Broad host range cloning vector, confers Km resistance | |
pER1 | pBBR1MCS-2 with phaZ1 inserted into Kpn1/Xba1 restriction sites, confers Km resistance | This study |
pER2 | pBBR1MCS-2 with phaZ2 inserted into Kpn1/Xba1 restriction sites, confers Km resistance | This study |
pER3 | pBBR1MCS-2 with phaZ3 inserted into Sal1/Xba1 restriction sites, confers Km resistance | This study |
pER4 | pBBR1MCS-2 with phaZ5 inserted into Kpn1/Xba1 restriction sites, confers Km resistance | This study |
pGY46 | phaC precise-deletion gene replacement plasmid, confers Km resistance | ([York et al. 2001]) |
pCJB3 | pGY46 with regions upstream and downstram of phaZ3 inserted between SacI and XbaI | This study |