|
R. eutropha
strains
|
|
H16
|
Wild type, Gm resistant
|
ATCC 17699
|
|
Re1097
|
H16/ΔphaZ1
|
([York et al. 2003])
|
|
Re1107
|
H16/ΔphaZ5
|
([York et al. 2003])
|
|
Re1110
|
H16/ΔphaZ2
|
([York et al. 2003])
|
|
Re1111
|
H16/ΔphaZ1 ΔphaZ ΔphaZ5
|
([York et al. 2003])
|
|
Re1112
|
H16/ΔphaZ1 ΔphaZ2
|
([York et al. 2003])
|
|
Re2005
|
H16/ΔphaZ1 ΔphaZ2 ΔphaZ3 ΔphaZ5
|
This study
|
|
Re2006
|
H16/ΔphaZ3
|
This study
|
|
E. coli
strains
|
|
Top10
|
IF-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZ ΔM15 ΔlacX 74 recA 1 araD 139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA 1 nupG λ-
|
Invitrogen
|
|
S17-1
|
recA pro hsdR RP4-2-Tc::Mu-Km::Tn7
|
ATCC47055; ([Simon et al. 1983])
|
|
Plasmids
|
|
pBBR1MCS-2
|
Broad host range cloning vector, confers Km resistance
|
([Kovach et al. 1995])
|
|
pER1
|
pBBR1MCS-2 with phaZ1 inserted into Kpn1/Xba1 restriction sites, confers Km resistance
|
This study
|
|
pER2
|
pBBR1MCS-2 with phaZ2 inserted into Kpn1/Xba1 restriction sites, confers Km resistance
|
This study
|
|
pER3
|
pBBR1MCS-2 with phaZ3 inserted into Sal1/Xba1 restriction sites, confers Km resistance
|
This study
|
|
pER4
|
pBBR1MCS-2 with phaZ5 inserted into Kpn1/Xba1 restriction sites, confers Km resistance
|
This study
|
|
pGY46
|
phaC precise-deletion gene replacement plasmid, confers Km resistance
|
([York et al. 2001])
|
|
pCJB3
|
pGY46 with regions upstream and downstram of phaZ3 inserted between SacI and XbaI
|
This study
|
