Examination of PHB Depolymerases in Ralstonia eutropha: Further Elucidation of the Roles of Enzymes in PHB Homeostasis

AMB Express

Table 1 R. eutropha PHA depolymerase and related enzymes

Name	Function	PHA Depolymerase Superfamily designation^a	Protein ID^b	Locus tag
PhaZ1	Intracellular PHA depolymerase	i-nPHAscl (no lipase box)	CAJ92291.1	H16_A1150
PhaZ2	Intracellular PHA depolymerase	i-nPHAscl (no lipase box)	CAJ93939.1	H16_A2862
PhaZ3	Putative intracellular PHA depolymerase	i-nPHAscl (no lipase box)	CAJ95139.1	H16_B0339
PhaZ4	Putative PHA depolymerase	i-nPHAscl (no lipase box)	AAP85930.1	PHG178
PhaZ5	Intracellular PHA depolymerase	i-nPHAscl (no lipase box)	CAJ95805.1	H16_B1014
PhaZ6^c	PHA depolymerase	e-dPHAscl (catalytic domain type 1)	CAJ96855.1	H16_B2073
PhaZ7	PHA depolymerase	e-dPHAscl (catalytic domain type 1)	CAJ97183.1	H16_B2401
PhaY1	D-(−)-3-hydroxybutyrate oligomer hydrolase	N/A^d	CAJ93348.1	H16_A2251
PhaY2	D-(−)-3-hydroxybutyrate oligomer hydrolase	N/A^d	CAJ92475.1	H16_A1335

^aPHA Depolymerase Superfamily designation according to the PHA Depolymerase Engineering Database (http://www.ded.uni-stuttgart.de/). Putative primary protein sequences of each PHA depolymerase were used as seed sequences for a BLAST search of enzymes present in the database. Each depolymerase candidate is also classified according to a superfamily, as discussed in ([Knoll et al. 2009]).
^bFrom DNA Data Bank of Japan (http://www.ddbj.nig.ac.jp/).
^cOriginally characterized as PhaZd ([Abe et al. 2005]).
^dSequence characteristics not present in PHA Depolymerase Engineering Database.