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AMB Express
Figure 1 | AMB Express

Figure 1

From: In vitro production of two chitinolytic proteins with an inhibiting effect on the insect coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae) and the fungus Hemileia vastatrix the most limiting pests of coffee crops

Figure 1

Exochitinase protein fractions on SDS-PAGE in 4-10% polyacrylamide. Lane 1. Bio-Rad dual molecular weight marker (range 250-10 kDa). Lane 2. Raw induced MBP/exochitinase (81 kDa) obtained by sonication of the bacteria. Lane 3. MBP/exochitinase cleaving reaction with Factor Xa protease. MBP (50 kDa) and exochitinase (31 kDa) Lane 4. Cleavaging control (MBP/paramyosin) with factor Xa. Paramyosin (27.7 kDa). Lanes 5, 6, and 7. Fractions of pure exochitinase obtained from the cleavage of MBP/exochitinase and separated by ion-exchange chromatography. Lanes 8, 9, and 10. Fractions of MBP alone obtained from the cleavage of MBP/exochitinase and separated by ion-exchange chromatography.

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