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AMB Express

Table 2 Purification of the recombinant MLE.

From: Heterologous expression of Oenococcus oeni malolactic enzyme in Lactobacillus plantarum for improved malolactic fermentation

Purification step

Total activity (U)

Total protein

(mg)

Specific activity (U/mg)

Purification (fold)

Recovery (%)

Crude extract

3,289

148.7

22.1

1.0

100

Affinity chromatography

1,748

8.05

217

9.8

53

  1. The MLE was expressed in 0.5 litre and values are the mean of duplicate experiments
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