Heterologous expression of Oenococcus oeni malolactic enzyme in Lactobacillus plantarum for improved malolactic fermentation

AMB Express

Table 1 Overview of recent work of MLE production in recombinant Escherichia coli, Lactobacillus plantarum, Saccharomyces cerevisiae and Schizosaccharomyces pombe.

Source of *mle*	Expression host	L-malic acid degradation (g/l per day)	Specific activity of crude enzyme (U/mg)	References
Lactobacillus delbrueckii	Escherichia coli	0.05	ND	Williams et al. (1984)
	Saccharomyces cerevisiae	0.01	ND
Lactococcus lactis	Escherichia coli	0.23	ND	Ansanay et al. (1993)
	Saccharomyces cerevisiae	0.39 (pH 3.0)	ND
	Escherichia coli	ND	0.27	Denayrolles et al. (1994)
	Saccharomyces cerevisiae	0.08 (pH 3.0)	0.7	Denayrolles et al. (1995)
	Saccharomyces cerevisiae	0.14 (pH 3.5)	18	Ansanay et al. (1996)
	Schizosaccharomyces pombe	1.50*,⁺ (pH 3.5)	ND
	Saccharomyces cerevisiae	0.72⁺ (pH 3.5)	ND	Bony et al. (1997)
Pediococcus damnosus	Saccharomyces cerevisiae	2.00⁺ (pH 3.3)	ND	Bauer et al. (2005)
	Escherichia coli	Not detectable	0.13**	Labarre et al. (1996)
Oenococcus oeni	Saccharomyces cerevisiae	1,05 (pH 3.0)	0.02	Labarre et al. (1996)
	Saccharomyces cerevisiae	1.81⁺ (pH 3.5)	ND	Husnik et al. (2007)
	Saccharomyces cerevisiae	0.34	ND	Liu and Li (2009)
	Escherichia coli	ND	14.9	Schümann (personal communication))
	Lactobacillus plantarum	5.0*,⁺ (pH 4.0)	22.1	This work

Summary of available data on the consumption of L-malic acid (g/l) per day from medium, pH is indicated in parenthesis. The specific activities of the crude extracts (CE) are presented in μmol/ml per min and mg protein (U/mg). Schizosaccharomyces pombe
ND, not determined
*conversion of malic acid similar to the control
**after ammonium sulfate precipitation
⁺complete conversion of malic acid