Figure 4

Conversion of L-malic acid in malolactic test solution. The wild-type (dashed line) and recombinant L. plantarum (solid line) strain were incubated at 25°C in solution containing 3.0 g/L (100%) L-malic acid (pH 5.0). The conversion is displayed without addition (○) and with addition of 0.1 mM NAD⁺ and 0.02 mM Mn²⁺ (●). (A) shows the time course of malic acid conversion and (B) presents the conversion of L-malic acid in relation to the cell density. All experiments were carried out in triplicate.