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AMB Express
Figure 1 | AMB Express

Figure 1

From: Exploiting the inter-strain divergence of Fusarium oxysporum for microbial bioprocessing of lignocellulose to bioethanol

Figure 1

Genetic diversity among eight strains of Fusarium oxysporum , based on DNA sequence data from fragments of the (A) internal transcribed spacer (ITS) region of nrDNA (B) elongation factor-1 alpha ( EF-1α ) gene. Sequences analysed were derivedfrom F. oxysporum strains 4E, 7E, 12A, 13C, 11C, 13B, 21D, 27E or F. oxysporum sequences within the NCBI Nucleotide database (http://www.ncbi.nlm.nih.gov/nuccore): F-X.1.7-030520-03 (GenBank No. EU364857.1), FO-10 (GenBank No. AY928417.1), FoChz10c (GenBank No. EU313533.1) and NRRL 38291 (GenBank No. FJ985370.1). The ITS was amplified using PCR primers ITS4 and ITS5 as described by White et al. (White et al. 1990). The 5' portion of EF-1α was amplified using the primers EF-1 and EF-2 as described by O'Donnell et al. (1998). DNA sequences were aligned using European Bioinformatics Institutes's ClustalW2 tool (www.ebi.ac.uk) (Larkin et al. 2007) and phylogenetic trees were generated using the Neighbor-joining method (Saitou & Nei 1987)

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