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AMB Express

Table 1 Primer pairs of synthetic oligonucleotide designed

From: Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1

Primer

Oligonucleotide sequence

FP4

5'-CTGGTTGATYCTGCCAGT-3'

RP1438

5'-GGGCATCACAGACCTGTTAT-3'

DFP

5'-AAYATGGAYATHGAYTGYGA-3'

DRP

5'-RTCDCCCCADATNCCRTA-3'

FP1

5-CCAGAGCACGTTGGCATCAA-3

RP1

5-ACCATAGTCGGACTTGACCT-3

FP2

5'-GGTCTGCAACAACAAGCTCATC-3'

RP2

5'-GAGTCGATGCCGTCTTGATC-3'

csn -f

5'-CGCCATATG AAAACAGCTGCCATT-3

csn -F

5'- CGCCATATG TACGATATCCCTGACAAC-3'

csn -r

5'-ATTCTCGAG TTACAGAGACGCAACAAG

  1. The primers, csn-f and csn-F were designed with a Nde I site, while csn-r was with Xho I site, which are underlined are underlined, respectively.
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