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Table 1 Primer pairs of synthetic oligonucleotide designed

From: Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1

Primer Oligonucleotide sequence
FP4 5'-CTGGTTGATYCTGCCAGT-3'
RP1438 5'-GGGCATCACAGACCTGTTAT-3'
DFP 5'-AAYATGGAYATHGAYTGYGA-3'
DRP 5'-RTCDCCCCADATNCCRTA-3'
FP1 5-CCAGAGCACGTTGGCATCAA-3
RP1 5-ACCATAGTCGGACTTGACCT-3
FP2 5'-GGTCTGCAACAACAAGCTCATC-3'
RP2 5'-GAGTCGATGCCGTCTTGATC-3'
csn -f 5'-CGCCATATG AAAACAGCTGCCATT-3
csn -F 5'- CGCCATATG TACGATATCCCTGACAAC-3'
csn -r 5'-ATTCTCGAG TTACAGAGACGCAACAAG
  1. The primers, csn-f and csn-F were designed with a Nde I site, while csn-r was with Xho I site, which are underlined are underlined, respectively.