Figure 5

Phylogenetic sequences of GH75 chitosanases from 10 fungal proteins. The Phylogenetic tree was constructed with Mega 4. Neighbor-joining was used with 1000 random bootstrap replication. Alignment of the GH75 domain amino acid sequences used Clustal X (1.83). A bar represents the evolutionary distance of 0.05. The DDBJ/EMBL/GenBank accession numbers are shown in parentheses. The multiple sequence alignment was performed using Clustal X program (Jeanmougin et al. 1998) and visually examined with BoxShade Server program (http://www.ch.embnet.org/software/BOX_form.html).