Effects of temperature on the activity (◆). The enzyme activities were assayed under standard conditions at various temperatures from 36 to 64°C. Stability of the chitosanases (▲). The enzymes were incubated for 60 min at various temperatures and the residual activities were assayed under standard conditions. The highest residual chitosanase activity, which was dealt with after 1 h at 0 degC at pH4, was setted 100%.