Effects of pH on the activity (◆). The enzyme activities were measured under standard assay conditions with various buffers at pH 2.5 to 7.0. Stability of the chitosanases (▲). The enzymes were incubated for 60 min at 37°C in various buffers at pH 2.5 to 8.0 and the residual activities were assayed under standard conditions. The buffers used were McIlvains buffer. The highest residual chitosanase activity, which was dealt with after 1 h at 37 degC at pH4, was setted 100%.