Figure 2From: Cloning and overexpression of a new chitosanase gene from Penicillium sp. D-1SDS-PAGE analysis of the recombinant CSN overexpressed in E. coli BL21 (DE3). Lane M, protein size markers. The sizes of protein markers are indicated. Lane 1, the supernatant of sonicated cell suspension. Lane2, the total cell lysate. Lane 3, purified CSN by affinity chromatography of Ni2+-NTA.Back to article page