Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology

AMB Express

Table 1 Induction parameters screened for bromelain production in BL21-AI

Induction condition¹	Range²
Cell concentration (induction time)	OD_{600 nm} of 0.4 - 0.8
L-arabinose concentration	0.1 - 0.3% (w/v)
Post-induction period (harvest time)	2 - 10 hr
Temperature	20 - 37°C

¹All the tests were carried out at 37°C, OD_{600 nm} of 0.4, 0.2% (w/v) L-arabinose and 4 hr post- induction period (besides the specific test conditions).
²Cultivation conditions and their ranges were selected based on literature reports on recombinant protein expression.