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Table 1 Induction parameters screened for bromelain production in BL21-AI

From: Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology

Induction condition1 Range2
Cell concentration (induction time) OD600 nm of 0.4 - 0.8
L-arabinose concentration 0.1 - 0.3% (w/v)
Post-induction period (harvest time) 2 - 10 hr
Temperature 20 - 37°C
  1. 1All the tests were carried out at 37°C, OD600 nm of 0.4, 0.2% (w/v) L-arabinose and 4 hr post- induction period (besides the specific test conditions).
  2. 2Cultivation conditions and their ranges were selected based on literature reports on recombinant protein expression.