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Figure 5 | AMB Express

Figure 5

From: Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology

Figure 5

The 3D response surface curves of the combined effects of post-induction temperature, inducer concentration and post-induction period on bromelain production. (a) Temperature and inducer concentration at a fixed level of post-induction period. Tests were carried out at OD600 nm of 0.6 with varying temperature (20-30°C) and L-arabinose concentrations (0.1-0.3% w/v). Post-induction period of 8 hr was used. (b) Post-induction temperature and post-induction period at fixed level of inducer concentration. Tests were carried out at OD600 nm of 0.6 with varying post-induction temperature (20-30°C) and post-induction period (2-10 hr). Inducer concentration of 0.2% (w/v) was used (c) Inducer concentration and post-induction period at fixed level of post-induction temperature. Tests were carried out at OD600 nm of 0.6 with varying L-arabinose concentrations (0.1-0.3% w/v) and post-induction period (2-10 hr). A post-induction temperature of 25°C was used.

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