TY - JOUR AU - Muntari, Bala AU - Amid, Azura AU - Mel, Maizirwan AU - Jami, Mohammed S. AU - Salleh, Hamzah M. PY - 2012 DA - 2012/02/15 TI - Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology JO - AMB Express SP - 12 VL - 2 IS - 1 AB - Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surface methodology using face centered central composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of R2 (0.989) showed that the quadratic model used for the prediction was highly significant (p < 0.05). Under the optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively developed cultivation conditions for better production of recombinant bromelain in shake flask culture. SN - 2191-0855 UR - https://doi.org/10.1186/2191-0855-2-12 DO - 10.1186/2191-0855-2-12 ID - Muntari2012 ER -