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Table 3 Box Behnken factorial experimental design representing response of phytase activity (U ml-1) as influenced by shaking speed and concentration of yeast extract and ammonium sulphate

From: Heterologous expression and optimization using experimental designs allowed highly efficient production of the PHY US417 phytase in Bacillus subtilis 168

Exp X1 X2 X3 Phytase activity (U ml-1) Phytase activity predicted (U ml-1)
1 300 5 10 35.73 ± 0.17 35.18
2 300 15 10 42.92 ± 0.21 43.36
3 250 5 5 30.15 ± 0.15 29.86
4 250 5 15 33.10 ± 0.13 34.43
5 250 15 5 34.51 ± 0.2 32.79
6 250 15 15 35.79 ± 0.17 37.37
7 200 10 5 17.51 ± 0.08 18.75
8 300 10 5 38.62 ± 0.19 39.89
9 200 10 15 24.57 ± 0.12 23.33
10 300 10 15 45.63 ± 0.36 44.47
11 250 10 10 34.36 ± 0.17 36.52
12 250 10 10 34.93 ± 0.10 36.52
13 250 10 10 41.17 ± 0.23 36.52