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Table 2 Plackett-Burman design for 15 variables with coded values along with the observed results for phytase production

From: Heterologous expression and optimization using experimental designs allowed highly efficient production of the PHY US417 phytase in Bacillus subtilis 168

Trial A B C D E F G H I J K L M N O Phytase activity
(U ml-1)
1 + - - - + - - + + - + - + + + 3.96 ± 0.01
2 + + - - - + - - + + - + - + + 13.39 ± 0.34
3 + + + - - - + - - + + - + - + 27.20 ± 0.62
4 + + + + - - - + - - + + - + - 28.18 ± 0.70
5 - + + + + - - - + - - + + - + 24.58 ± 0.53
6 + - + + + + - - - + - - + + - 7.52 ± 0.18
7 - + - + + + + - - - + - - + + 11.68 ± 0.30
8 + - + - + + + + - - - + - - + 23.60 ± 0.59
9 + + - + - + + + + - - - + - - 10.80 ± 0.27
10 - + + - + - + + + + - - - + - 9.84 ± 0.25
11 - - + + - + - + + + + - - - + 26.01 ± 0.65
12 + - - + + - + - + + + + - - - 10.68 ± 0.26
13 - + - - + + - + - + + + + - - 7.45 ± 0.19
14 - - + - - + + - + - + + + + - 26.64 ± 0.62
15 - - - + - - + + - + - + + + + 0.94 ± 0.02
16 - - - - - - - - - - - - - - - 11.17 ± 0.40
E(Xi) 0.88 2.83 12..94 -0.36 -5.63 1.32 -0.11 -2.76 1.02 -4.70 5.00 3.41 -3.18 -4.92 2.38