Heterologous expression and optimization using experimental designs allowed highly efficient production of the PHY US417 phytase in Bacillus subtilis 168

AMB Express

Table 2 Plackett-Burman design for 15 variables with coded values along with the observed results for phytase production

Trial	A	B	C	D	E	F	G	H	I	J	K	L	M	N	O	Phytase activity (U ml^-1)
1	+	-	-	-	+	-	-	+	+	-	+	-	+	+	+	3.96 ± 0.01
2	+	+	-	-	-	+	-	-	+	+	-	+	-	+	+	13.39 ± 0.34
3	+	+	+	-	-	-	+	-	-	+	+	-	+	-	+	27.20 ± 0.62
4	+	+	+	+	-	-	-	+	-	-	+	+	-	+	-	28.18 ± 0.70
5	-	+	+	+	+	-	-	-	+	-	-	+	+	-	+	24.58 ± 0.53
6	+	-	+	+	+	+	-	-	-	+	-	-	+	+	-	7.52 ± 0.18
7	-	+	-	+	+	+	+	-	-	-	+	-	-	+	+	11.68 ± 0.30
8	+	-	+	-	+	+	+	+	-	-	-	+	-	-	+	23.60 ± 0.59
9	+	+	-	+	-	+	+	+	+	-	-	-	+	-	-	10.80 ± 0.27
10	-	+	+	-	+	-	+	+	+	+	-	-	-	+	-	9.84 ± 0.25
11	-	-	+	+	-	+	-	+	+	+	+	-	-	-	+	26.01 ± 0.65
12	+	-	-	+	+	-	+	-	+	+	+	+	-	-	-	10.68 ± 0.26
13	-	+	-	-	+	+	-	+	-	+	+	+	+	-	-	7.45 ± 0.19
14	-	-	+	-	-	+	+	-	+	-	+	+	+	+	-	26.64 ± 0.62
15	-	-	-	+	-	-	+	+	-	+	-	+	+	+	+	0.94 ± 0.02
16	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	11.17 ± 0.40
E(Xi)	0.88	2.83	12..94	-0.36	-5.63	1.32	-0.11	-2.76	1.02	-4.70	5.00	3.41	-3.18	-4.92	2.38