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Table 1 Assigned concentrations of different parameters and their levels in Plackett-Burman design for phytase production

From: Heterologous expression and optimization using experimental designs allowed highly efficient production of the PHY US417 phytase in Bacillus subtilis 168

Codes Factors Level (-1) Level (+1)
A T 30 37
B pH 5.5 7.5
C Shaking speed (rpm) 150 250
D Inoculum size (OD600f) 0.05 0.5
E KH2PO4 0 5
F Methanol 0 5
G Glycerol 0 5
H Galactose 0 5
I Urea 0 5
J Casein hydrolysate 0 5
K Yeast extract 0 5
L (NH4)2 SO4 0 5
M Triton X-100 0 5
N Phytic acid 0 0.01
O Corn steep liquor 0 10