Figure 1From: Heterologous expression and optimization using experimental designs allowed highly efficient production of the PHY US417 phytase in Bacillus subtilis 168Detection of phytase activity in Bacillus subtilis 168 transformants. Colonies of B. subtilis 168 carrying pAF3 (a) and pMSP3535 (b) were grown on LB agar supplemented with 3 mM of phytic acid for 24 h and then phytase activity has been visualized by the two step counterstaining treatment.Back to article page